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The fixation and subsequent immunostaining were performed as in other experiments (see Fixation and post-fixation immunostaining), using 1:250 rabbit monoclonal anti-His-Tag (#12698

doi: 10.1016/j.anifeedsci.2021.114946 72 de Vries JYPundirSMckenzieEKeijerJKussmannM

Together, these observations encouraged us to further explore ML210 as a potential direct and selective inhibitor of GPX4 with a novel mechanism of action